Turn off the power and disconnect the cable before attempting any maintenance.
Insert the new lamp, tighten the screws, and reconnect the power cable. Reset the lamp usage hours in the maintenance menu. Flow Cell Cleaning
Pass an appropriate solvent (like methanol or acetonitrile) through the flow cell at a low flow rate. shimadzu spd20a user manual
Pump your mobile phase through the system at the analytical flow rate. Monitor the baseline until it is visually flat. Press the to re-zero the detector. 5. Maintenance and Component Replacement
Proper maintenance requires periodic lamp replacement. The manual explains how to safely access the deuterium ( D2cap D sub 2 ) lamp and, on the , the tungsten (W) lamp. Turn off the power and disconnect the cable
The SPD-20A/AV includes a solvent recycling valve option ( SV.WASTE ). This component can be programmed to divert the initial flow of solvent (which may contain impurities) to waste and then switch to send the rest to a collection vessel for recycling. This feature helps reduce solvent consumption and operating costs.
For complete operational safety and detailed technical procedures, always refer to the official Shimadzu SPD-20A user manual provided with the instrument. Flow Cell Cleaning Pass an appropriate solvent (like
Comprehensive Guide to the Shimadzu SPD-20A UV-VIS Detector User Manual
: This occurs when the leak sensor detects liquid inside the instrument housing. Action : Immediately turn off the HPLC pump. Check all connections and unions for visible leaks. Tighten or replace fittings as necessary. If no leak is visible, the sensor may be too sensitive; the LEAK THR setting may need to be adjusted.
Turn off the power and disconnect the cable before attempting any maintenance.
Insert the new lamp, tighten the screws, and reconnect the power cable. Reset the lamp usage hours in the maintenance menu. Flow Cell Cleaning
Pass an appropriate solvent (like methanol or acetonitrile) through the flow cell at a low flow rate.
Pump your mobile phase through the system at the analytical flow rate. Monitor the baseline until it is visually flat. Press the to re-zero the detector. 5. Maintenance and Component Replacement
Proper maintenance requires periodic lamp replacement. The manual explains how to safely access the deuterium ( D2cap D sub 2 ) lamp and, on the , the tungsten (W) lamp.
The SPD-20A/AV includes a solvent recycling valve option ( SV.WASTE ). This component can be programmed to divert the initial flow of solvent (which may contain impurities) to waste and then switch to send the rest to a collection vessel for recycling. This feature helps reduce solvent consumption and operating costs.
For complete operational safety and detailed technical procedures, always refer to the official Shimadzu SPD-20A user manual provided with the instrument.
Comprehensive Guide to the Shimadzu SPD-20A UV-VIS Detector User Manual
: This occurs when the leak sensor detects liquid inside the instrument housing. Action : Immediately turn off the HPLC pump. Check all connections and unions for visible leaks. Tighten or replace fittings as necessary. If no leak is visible, the sensor may be too sensitive; the LEAK THR setting may need to be adjusted.