Are you interested in the (e.g., nanopore vs. sequencing-by-synthesis) used by this device?
As sequencing begins, watch the real-time quality metric dashboards. The device will dynamically display read counts, Q-scores, and identified taxa. Step 5: Data Export and Synchronization
The use of next-generation sequencing technologies for ... - IRIS
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Weighing just under 450 grams (approx. 1 lb), the ergonomic chassis easily fits into a standard field jacket pocket or tactical backpack. portable sequencher 414
| Metric | Sequencher 414 | MinION Mk1C | Flongle | |--------|----------------|-------------|---------| | Active pores | 414 | 512 (but typical 200–400) | 126 | | Throughput (72h) | 25 Gb | 15 Gb | 2.5 Gb | | Power (W) | 3.5 (peak) | 12 | 2.5 (USB) | | Weight (g) | 180 | 350 (with screen) | 15 (dongle) | | On-device basecalling | Yes (ASIC) | Yes (CPU/GPU) | No (host required) | | Adaptive sampling | Per-pore RL | Bulk voltage reversal | No |
Hit "Start." The device will automatically perform sample lysis, library prep, and begin sequencing. Live data, including read counts and species identification mapping, will populate on the screen instantly.
Fieldwork is rarely clean or dry. The Sequencher 414 features an IP65 rating, making it completely dust-tight and resistant to water splashes from any direction. The internal cartridge bay is thermally regulated, allowing the device to operate flawlessly in ambient temperatures ranging from -10°C to 45°C (14°F to 113°F). Automated Sample Preparation
Sample preparation has historically been the bottleneck of field genomics. The 414 utilizes automated, all-in-one microfluidic cartridges. Users simply load a raw sample—such as blood, water, or plant tissue—into the cartridge. The device automatically handles cell lysis, DNA extraction, and library preparation without requiring pipettes or thermal cyclers. 3. Onboard AI and Edge Computing Are you interested in the (e
Pores are grouped into 6 independent fluidic cells (69 pores each) to allow multiplexed sample loading.
Farmers can test livestock or crops for diseases right in the field. Identifying a fungal blight or swine fever strain within an hour protects food supplies and saves millions of dollars in livestock losses. Advantages Over Traditional Laboratory Sequencing Traditional Lab Sequencers Portable Sequencher 414 Fixed benchtop environment Anywhere (Field, clinic, space) Time to Result days to weeks 30 to 120 minutes Upfront Cost $50,000 - $500,000+ Affordable entry-tier pricing Infrastructure Requires cleanrooms and stable power Runs on battery power Challenges and Future Outlook
: Automatically converts bulky SAM files to compact BAM files and generates publication-ready graphics for RNA-Seq. Sequencher Features | DNA Sequencing Software
Use the file menu to import raw chromatogram files (e.g., .ab1 files). The device will dynamically display read counts, Q-scores,
[Raw Electrical Fluctuations] │ (Local Basecalling) ▼ [FASTQ Sequence Data] │ (Quality & Primer Trimming) ▼ [High-Confidence Filtered Reads] 4. Alignment and Consensus Generation
Field research often means working entirely offline. The 414 features an onboard ARM-based neural processing unit (NPU). This processor handles raw signal processing, basecalling, and basic alignment locally, bypassing the need for an active internet or cloud connection. Hardware Specifications Matrix Specification 22.5 cm x 14.2 cm x 6.8 cm Weight 1.45 kg (including internal battery) Throughput Up to 15 Gb per 24-hour run Read Lengths
During the 2024 Marburg virus outbreak in Rwanda, field teams used Portable Sequencher 414 units to sequence viral genomes directly from patient blood samples without needing a cold chain for reagents (nanopore chemistry is stable at 4°C for weeks). The "414" channel density allowed them to sequence 24 samples simultaneously on a single flow cell.
IP67 rating (dust‑tight, waterproof to 1 m). Meets MIL‑STD‑810H for drop, vibration, and extreme temperature (-20°C to 50°C).